A team of conservation geneticists studied two isolated populations of the Indian rock python (Python molurus) — Population A (in a large continuous forest reserve, n = 2000) and Population B (on a small island, n = 40, colonised ~200 years ago by a small founding group of 8 individuals). Blood samples were analysed for a gene locus controlling scale pigmentation. Two alleles exist: D (dark pigmentation, dominant) and d (light pigmentation, recessive). In Population A, the frequency of light-pigmented individuals was found to be 16%. In Population B, all individuals showed dark pigmentation, but DNA sequencing revealed that 100% of individuals were heterozygous (Dd) at this locus.
Read the following passage and answer the questions that follow:
A team of conservation geneticists studied two isolated populations of the Indian rock python (Python molurus) — Population A (in a large continuous forest reserve, n = 2000) and Population B (on a small island, n = 40, colonised ~200 years ago by a small founding group of 8 individuals). Blood samples were analysed for a gene locus controlling scale pigmentation. Two alleles exist: D (dark pigmentation, dominant) and d (light pigmentation, recessive). In Population A, the frequency of light-pigmented individuals was found to be 16%. In Population B, all individuals showed dark pigmentation, but DNA sequencing revealed that 100% of individuals were heterozygous (Dd) at this locus.
(a) Using the Hardy-Weinberg principle, calculate the frequencies of alleles D and d, and the frequencies of genotypes DD, Dd, and dd in Population A. Show all working. (2 marks)
(b) Population B shows NO light-pigmented individuals despite carrying the d allele. Identify the evolutionary mechanism most likely responsible for the allele frequency difference between Population A and Population B. Justify your answer using TWO specific features of Population B described in the passage. (1 mark)
(c) Over the next 50 generations, researchers predict that Population B will show significantly reduced fitness due to 'inbreeding depression'. Explain the biological basis of this prediction. (1 mark)
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Part (a): Hardy-Weinberg calculation for Population A [2 marks]
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Step 1 — State the Hardy-Weinberg equation:
Let p = frequency of allele D (dominant)
Let q = frequency of allele d (recessive)
p² + 2pq + q² = 1 and p + q = 1
Step 2 — Find q from the given data:
Frequency of light-pigmented individuals = frequency of dd genotype = q²
q² = 16% = 0.16
q = √0.16 = 0.4
Step 3 — Find p:
p = 1 − q = 1 − 0.4 = 0.6
Step 4 — Calculate genotype frequencies:
• Frequency of DD (homozygous dominant) = p² = (0.6)² = 0.36 (i.e., 36%)
• Frequency of Dd (heterozygous) = 2pq = 2 × 0.6 × 0.4 = 0.48 (i.e., 48%)
• Frequency of dd (homozygous recessive) = q² = (0.4)² = 0.16 (i.e., 16%)
Verification: 0.36 + 0.48 + 0.16 = 1.00 ✓
[Award 1 mark for correct allele frequencies: q = 0.4, p = 0.6]
[Award 1 mark for correct genotype frequencies: DD = 0.36, Dd = 0.48, dd = 0.16]
(1 + 1 = 2 marks)
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Part (b): Evolutionary mechanism + Justification [1 mark]
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Mechanism: Genetic Drift — specifically the Founder Effect.
Justification using TWO features from the passage:
(i) Population B was founded by only 8 individuals — a very small founding group that carried only a fraction of the allele frequencies present in the original population. The d allele happened to be present only in heterozygous form (Dd) in these founders, so the dd genotype was never established in the island population.
(ii) The current population size remains very small (n = 40) — in small populations, random changes in allele frequency are large relative to population size, and allele frequencies can diverge drastically from the source population by chance alone, NOT due to natural selection.
(Any TWO of the above justification points accepted for full credit)
[Award 1 mark for: naming Genetic Drift / Founder Effect AND giving at least ONE valid feature-based justification from the passage]
(1 mark)
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Part (c): Biological basis of inbreeding depression [1 mark]
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Biological basis:
In a small, isolated population such as Population B, all individuals are closely related (descended from only 8 founders). Repeated mating among relatives (inbreeding) increases homozygosity at all gene loci across the genome.
As homozygosity increases, harmful recessive alleles (which were previously 'hidden' in heterozygotes) are increasingly expressed in the homozygous recessive condition.
This leads to reduced survival, reduced fertility, and increased susceptibility to disease — collectively called inbreeding depression — which lowers the overall fitness of the population.
[Award 1 mark for: stating that inbreeding increases homozygosity → harmful recessive alleles are expressed → reduced fitness / any equivalent correct explanation]
(1 mark)
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TOTAL: 2 + 1 + 1 = 4 marks