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TGBIE · 2nd Year · BiPC

Botany — Last-Minute Revision

High-priority revision for the TS Inter 2nd Year Botany board exam — high-yield units, must-know diagrams, and exam-day strategy. Paper: 60 marks · 3 hours.

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High-Yield Units

  • Genetics — Mendel's laws, dihybrid cross, linkage, mutations (LAQ+SAQ)
  • Molecular Biology — DNA replication, transcription, translation (LAQ)
  • Biotechnology — recombinant DNA, PCR, gene cloning, Bt crops (LAQ+SAQ)
  • Plant Physiology — photosynthesis (C3/C4/CAM), respiration, transpiration (LAQ)
  • Microbiology — bacteria, viruses, mycology applications (SAQ)
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Diagrams Must-Know

  • Chloroplast ultrastructure — grana, stroma, thylakoid membrane
  • Mitosis and Meiosis stages (prophase, metaphase, anaphase, telophase)
  • Calvin cycle (C3 pathway) — RuBP, 3-PGA, G3P steps
  • DNA double helix structure with base pairing
  • Lac operon — inducer/repressor mechanism diagram
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Key Concepts to Re-read

  • Law of Segregation vs. Law of Independent Assortment — give examples
  • Difference between DNA replication and transcription
  • Restriction enzymes — palindromic sites, sticky ends, blunt ends
  • Eutrophication and biological magnification — definition + stages
  • Polymerase Chain Reaction (PCR) — 3 steps: denaturation, annealing, extension
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Common Mistakes

  • Don't confuse genotype and phenotype ratios in dihybrid crosses
  • C4 plants: mesophyll → bundle sheath (not the reverse)
  • PCR uses DNA polymerase, not RNA polymerase
  • Transgenic and GMO are related but not synonymous — state definitions carefully
  • In lac operon, absence of glucose activates; absence of lactose also affects

Exam-Day Tips

  • Label all diagrams fully — parts without labels lose marks
  • For genetic crosses, draw Punnett square clearly, state the ratio
  • Define terms before answering — one mark is often for the definition alone
  • For molecular biology, write the enzyme name with each step
  • Read 'explain', 'describe', 'compare' carefully — they ask for different things